Microbiology Lab Manual John P Harley 8th
PART ONE Microscopic Techniques 1. Bright-Field Light Microscope (Dark Image, Brighter Background) and Microscopic Measurement of Organisms 2.
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The Hanging Drop Slide and Bacterial Motility 3. Dark-Field Light Microscope: Bright Image, Dark Background 4.
Phase-Contrast Light Microscope 5. Fluorescence Microscope PART TWO Bacterial Morphology and Staining 6. Negative Staining 7. Smear Preparation and Simple Staining 8. Gram Stain 9. Acid-Fast Staining (Ziehl-Neelsen and Kinyoun) Procedures 10. Endospore Staining (Schaeffer-Fulton or Wirtz-Conklin) 11.
Capsule Staining 12. Flagella Staining: West and Difco’s SpotTest Methods PART THREE Basic Laboratory and Culture Techniques 13.
Sterilization and Microbiological Culture Media Preparation 14. Culture Transfer Instruments, Techniques, and Isolation and Maintenance of Pure Cultures 15. Spread-Plate Technique 16.
The Streak-Plate Technique: Selective and Differential Media 17. Pour-Plate Technique 18. Cultivation of Anaerobic Bacteria 19. Determination of Bacterial Numbers PART FOUR Biochemical Activities of Bacteria 20. Carbohydrates I: Fermentation and β-Galactosidase Activity 21. Carbohydrates II: Triple Sugar Iron Agar Test 22. Carbohydrates III: Starch Hydrolysis 23.
Lipids: Lipid Hydrolysis 24. Proteins, Amino Acids, and Enzymes I: Hydrogen Sulfide Production and Motility 25. Proteins, Amino Acids, and Enzymes II: The IMViC Tests 26. Proteins, Amino Acids, and Enzymes III: Casein Hydrolysis 27. Proteins, Amino Acids, and Enzymes IV: Gelatin Hydrolysis 28. Proteins, Amino Acids, and Enzymes V: Catalase Activity 29. Proteins, Amino Acids, and Enzymes VI: Coagulase and DNase Activity 30.
Proteins, Amino Acids, and Enzymes VII: Oxidase Test and Bioluminescence 31. Proteins, Amino Acids, and Enzymes VIII: Urease Activity 32. Proteins, Amino Acids, and Enzymes IX: Lysine and Ornithine Decarboxylase Tests 33. Proteins, Amino Acids, and Enzymes X: Phenylalanine Deamination 34.
Proteins, Amino Acids, and Enzymes XI: Nitrate Reduction PART FIVE Rapid Multitest Systems 35. The API® 20E System 36. The EnteroPluri-Test System PART SIX Unknown Identification 37. Using the First Edition of Bergey’s Manual of Systematic Bacteriology to Identify Bacteria 38.
General Unknown PART SEVEN Environmental Factors Affecting Growth of Microorganisms 39. Temperature 40. Osmotic Pressure 42.
The Effects of Chemical Agents on Bacteria I: Disinfectants and Other Antimicrobial Products 43. The Effects of Chemical Agents on Bacteria II: Antimicrobial Agents (Kirby-Bauer Method) and the Etest® 44. Hand Washing, Environmental Sampling, and Microbiological Monitoring 45. Determination of a Bacterial Growth Curve: Classical and Two-Hour Methods PART EIGHT Environmental and Food Microbiology 46. Standard Coliform Most Probable Number (MPN) Test and Colilert®-18 Coliform Test 47.
Membrane Filter Technique for Coliforms and Fecal Streptococci; KONFIRMTM Test for Fecal Coliforms 48. Isolation of Escherichia coli Bacteriophages from Sewage and Determining Bacteriophage Titers 49.
Enumeration of Soil Microorganisms 50. Bacterial Count of a Food Product 51. Examination of Milk for Bacteria PART NINE Medical Microbiology 52. Hemagglutination Reactions: Blood Groups 53. Isolation of Normal Microbiota from the Human Body 54.
Staphylococci 55. Pneumococci 56. Streptococci 57.
Neisseriae 58. Aerobic and Anaerobic Endospore-Forming Bacteria PART TEN Survey of Selected Eucaryotic Microorganisms 59. Fungi (Ascomycota; also Ascomycetes) I: Yeasts 60. Fungi (Eumycota) II: Zygomycota (Rhizopus), Ascomycota (Penicillium), and Basidiomycota(Agaricus) PART ELEVEN Microbial Genetics and Genomics 61. Bacterial Mutation 62.
Bacterial Transformation 63. Bacterial Conjugation: The Transfer of Antibiotic-Resistant Plasmids 64. Isolation of Genomic DNA from Baker’s Yeast (Saccharomyces cerevisiae) 65. Isolation and Purifi cation of Genomic DNA from Escherichia coli PART TWELVE Scientific Inquiry 66. Independent Group Projects 67.
PART ONE Microscopic Techniques 1. Bright-Field Light Microscope (Dark Image, Brighter Background) and Microscopic Measurement of Organisms 2. The Hanging Drop Slide and Bacterial Motility 3. Dark-Field Light Microscope: Bright Image, Dark Background 4. Phase-Contrast Light Microscope 5. Fluorescence Microscope PART TWO Bacterial Morphology and Staining 6. Negative Staining 7.
Smear Preparation and Simple Staining 8. Gram Stain 9.
Acid-Fast Staining (Ziehl-Neelsen and Kinyoun) Procedures 10. Endospore Staining (Schaeffer-Fulton or Wirtz-Conklin) 11. Capsule Staining 12. Flagella Staining: West and Difco’s SpotTest Methods PART THREE Basic Laboratory and Culture Techniques 13. Sterilization and Microbiological Culture Media Preparation 14.
Culture Transfer Instruments, Techniques, and Isolation and Maintenance of Pure Cultures 15. Spread-Plate Technique 16. The Streak-Plate Technique: Selective and Differential Media 17. Pour-Plate Technique 18. Cultivation of Anaerobic Bacteria 19. Determination of Bacterial Numbers PART FOUR Biochemical Activities of Bacteria 20.
Carbohydrates I: Fermentation and β-Galactosidase Activity 21. Carbohydrates II: Triple Sugar Iron Agar Test 22. Carbohydrates III: Starch Hydrolysis 23. Lipids: Lipid Hydrolysis 24.
Proteins, Amino Acids, and Enzymes I: Hydrogen Sulfide Production and Motility 25. Proteins, Amino Acids, and Enzymes II: The IMViC Tests 26. Proteins, Amino Acids, and Enzymes III: Casein Hydrolysis 27. Proteins, Amino Acids, and Enzymes IV: Gelatin Hydrolysis 28. Proteins, Amino Acids, and Enzymes V: Catalase Activity 29. Proteins, Amino Acids, and Enzymes VI: Coagulase and DNase Activity 30. Proteins, Amino Acids, and Enzymes VII: Oxidase Test and Bioluminescence 31.
Microbiology Lab Manual John P Harley 8th Grade
Proteins, Amino Acids, and Enzymes VIII: Urease Activity 32. Proteins, Amino Acids, and Enzymes IX: Lysine and Ornithine Decarboxylase Tests 33. Proteins, Amino Acids, and Enzymes X: Phenylalanine Deamination 34. Proteins, Amino Acids, and Enzymes XI: Nitrate Reduction PART FIVE Rapid Multitest Systems 35. The API® 20E System 36. The EnteroPluri-Test System PART SIX Unknown Identification 37.
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Using the First Edition of Bergey’s Manual of Systematic Bacteriology to Identify Bacteria 38. General Unknown PART SEVEN Environmental Factors Affecting Growth of Microorganisms 39. Temperature 40. Osmotic Pressure 42. The Effects of Chemical Agents on Bacteria I: Disinfectants and Other Antimicrobial Products 43. The Effects of Chemical Agents on Bacteria II: Antimicrobial Agents (Kirby-Bauer Method) and the Etest® 44. Hand Washing, Environmental Sampling, and Microbiological Monitoring 45.
Determination of a Bacterial Growth Curve: Classical and Two-Hour Methods PART EIGHT Environmental and Food Microbiology 46. Standard Coliform Most Probable Number (MPN) Test and Colilert®-18 Coliform Test 47. Membrane Filter Technique for Coliforms and Fecal Streptococci; KONFIRMTM Test for Fecal Coliforms 48. Isolation of Escherichia coli Bacteriophages from Sewage and Determining Bacteriophage Titers 49.
Enumeration of Soil Microorganisms 50. Bacterial Count of a Food Product 51. Examination of Milk for Bacteria PART NINE Medical Microbiology 52. Hemagglutination Reactions: Blood Groups 53. Isolation of Normal Microbiota from the Human Body 54. Staphylococci 55.
Pneumococci 56. Streptococci 57. Neisseriae 58. Aerobic and Anaerobic Endospore-Forming Bacteria PART TEN Survey of Selected Eucaryotic Microorganisms 59. Fungi (Ascomycota; also Ascomycetes) I: Yeasts 60. Fungi (Eumycota) II: Zygomycota (Rhizopus), Ascomycota (Penicillium), and Basidiomycota(Agaricus) PART ELEVEN Microbial Genetics and Genomics 61.
Bacterial Mutation 62. Bacterial Transformation 63.
Bacterial Conjugation: The Transfer of Antibiotic-Resistant Plasmids 64. Isolation of Genomic DNA from Baker’s Yeast (Saccharomyces cerevisiae) 65. Isolation and Purifi cation of Genomic DNA from Escherichia coli PART TWELVE Scientific Inquiry 66.
Independent Group Projects 67.