Axioskop 2 Manual
Brightfield Microscopy Use A. Setting up Koehler Illumination Transmitted-light brightfield microscopy allows for viewing of high-contrast or stained specimens. (Nonfluorescent specimens, e.g., H&E slides, alkaline phosphatase localization). PLEASE REFER TO FIG.
Knowledge of this manual is required for the operation of the instrument. The Axioplan 2 imaging Universal Microscope has been de. O Axioskop 2 MOT. Feb 1, 2013 - Axioskop 2 mot plus. B 40-075 e 10/03. Knowledge of this manual is required for the operation of the instrument. Would you therefore. Document Directory Database Online Manual For Zeiss Axioskop 2 Manual For Zeiss Axioskop 2 - In this site is not the same as a answer encyclopedia you buy in a autograph.
3-1 and KEY TO FIG. 3-1 FOR OPERATION AND FUNCTION CONTROLS To utilize the entire optical performance of the microscope, especially of the objective, the condenser, the luminous-field diaphragm and the aperture diaphragm should be set in accordance with the rules for KOEHLER illumination. After each objective change, field size and objective aperture change. THUS REPEAT STEPS 6-10. For objectives between 1.25X and 5X, the front lens of the condenser must be swung out (using lever 3-2/ 2) and the aperture diaphragm opened entirely (adjusting slider 3-2/ 3). TO AVOID GETTING HEADACHES, PLEASE MAKE THE FOLLOWING ADJUSTMENTS: Interpupillary Distance Adjust interpupillary distance by separate adjustment of the two eyepiece tubes (3-1/ 1). Diopter Adjustment While looking at a focused specimen through the eyepieces, use the diopter ring on each eyepiece to adjust separately for each eye.
Operation of the AxioVision software for brightfield microscopy. WHITE BALANCE: In order to achieve a color display which is neutral and close to reality it is necessary to balance the individual color channels against one another. For this, you need to use the pointer to click in the live image on an area of the microscope's specimen, which is white, neutral and illuminated as evenly as possible. Click on the WHITE BALANCE which automatically starts up a live image. It is important to set the exposure correctly (this can be carried out via automatic exposure measurement). Save your image in the archive or export the image in another file format using the function SAVE AS in the FILE menu. Save your images in your folder found in 'My Documents.'
Axioskop 2 Plus
NOTE:When saving images, you can select from one of two storage formats: Carl Zeiss Image Format (.zvi) In this format, important additional information, such as the scaling used, is stored with the image. All annotations are also stored separately from the image data. This allows them to be modified later. Other image format (NOT.zvi) If another image format (e.g., TIF, JPG) is selected for the images, only the pure image data are stored, without additional information and annotations. Annotations must then be burnt into the image (activate BURN IN ANNOTATIONS).
In this case, however, they cannot be subsequently modified. If you have used the 100X oil lens (available from Hilenski), clean the lens with LENS PAPER ONLY. Cover the microscope with the blue cover to keep out dust (Do not cover the Hg lamp if it is HOT). TO PUT A SCALE BAR ON YOUR MICROGRAPH:. Click SCALINGS on the workflow tab.
The SCALINGS property page is displayed. Select the tab sheet EDIT.
Select from the SCALING drop-down list the scaling of your choice (the objective you used to generate the image) and then click APPLY. The scaling has now been allocated to the image and is used for drawing in scale bars or for measurements. Click on the SCALE BAR icon to activate it. Then click on the image and drag the mouse to draw the appropriately-sized scale bar.
To change the characteristics of the scale bar, right click on the image and select PROPERTIES to activate the PROPERTIES window. Click on ATTRIBUTES. After selecting the scale bar in the image (click on the word SCALEBAR on the left side of the window to highlight), you can change the line color, line width, font and text color of the annotations in the scale bar. If you inadvertently add too many scale bars, highlight the ones you want to delete, go to EDIT and choose DELETE. To eliminate the text, select the text and simply drag it off the screen.
STORING YOUR DATA. You may store your data temporarily under 'My Documents.' This folder is unique to each user and is not shared. Other users do not have access to your files. Please transfer all your files to a CD or Zip disk preferably at the same session that you use the microscope.
The files stored on this computer will be deleted periodically to maintain the computer's optimal performance. IT IS YOUR RESPONSIBILITY TO SAVE YOUR DATA.
To use the CD creator program, click on the CREATE CD icon on the desktop and choose the data CD option. The files that are on this computer will show up on the top right quadrant.
Drag the files you want to record to the bottom quadrant. Once you have all the files there, click record. If you are only recording a small amount of data, you should choose the track-at-once option in the next screen and ensure that LEAVE CD OPEN is checked. FLUORESCENCE MICROSCOPY USE. NOTE: When re-lighting the lamp after it has been extinguished, allow the lamp to cool for approximately 10 minutes before turning it on again. SAFETY INSTRUCTIONS FOR Hg LAMP.
Do not look directly at the burning lamp without protective glasses. Do not expose bare skin to the radiation. Do not touch the lamp with your bare skin when it is hot. Do not put paper, cloth or any inflammable material near or over the lamp. Turn off the power when replacing the lamp or cleaning the lamp house.
When changing the lamp, do not touch the quartz glass envelope without gloves. If the lamp has been soiled with dirt or fingerprints, wipe the soiled area with an attached napkin or soft cloth soaked in alcohol. Replace the lamp when it has reached the end of its rated life (200 hours). Return the used lamp to qualified disposer or lamp vendor.
Install the lamp with correct polarity. IN CASE OF A LAMP BURST. 2017 ford f650 service manual.
In a rare accidental case of a lamp burst while it is on, to prevent inhaling the Hg fumes, leave the accident site immediately and ventilate the area or the room for at least 30 minutes. Call the Environmental Health and Safety Office at Emory at 404-727-5688.
If the mercury fumes have been inhaled, consult a physician for medical treatment. NOTE: When re-lighting the lamp after it has been extinguished, allow the lamp to cool for approximately 10 minutes before turning it on again. Save your image in the archive or export the image in another file format using the function SAVE AS in the FILE menu.
Save your images in your folder found in 'My Documents.' NOTE:When saving images, you can select from one of two storage formats: Carl Zeiss Image Format (.zvi) In this format, important additional information such as the scaling used is stored with the image. All annotations are also stored separately from the image data.
This allows them to be modified later. Other image format (NOT.zvi) If another image format (e.g., TIF, JPG) is selected for the images, only the pure image data are stored, without additional information and annotations. Annotations must then be burnt into the image (activate BURN IN ANNOTATIONS). In this case, however, they cannot be subsequently modified. At the end of your session:. Always exit the Axiovision program. Log off the computer.
Turn off the computer and monitor. Turn off the halogen lamp and the Hg lamp (if used). Unplug the Axiocam camera. Clean up the working area. If you have used the 100X oil lens (available from Hilenski), clean the lens with LENS PAPER ONLY.
Cover the microscope with the blue cover to keep out dust (Do not cover the Hg lamp if it is HOT). How to Take Multichannel Images in Zeiss AxioVision (Double or Triple Labeled Images).